KMID : 1007520220310070827
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Food Science and Biotechnology 2022 Volume.31 No. 7 p.827 ~ p.841
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Influence of thermophilic microorganism on non-volatile metabolites during high-temperature pile-fermentation of Chinese dark tea based on metabolomic analysis
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Zhu Wen
Wang Wenfeng Xu Wencan Wu Shuang Chen Wenjun Huang Youyi Wang Shengpeng
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Abstract
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Pile-fermentation is a critical procedure for producing Chinese dark tea, during which thermophilic microorganisms would play an irreplaceable role. However, there have been little researches on the influences of thermophilic microorganism pile-fermentation (TMPF) in high-temperature of Chinese dark tea. Thus, we conducted high-performance liquid chromatography and nontargeted metabolomic to analyze the non-volatile metabolites of TMPF. Our results discovered that the amounts of (??)-epigallocatechin gallate, (??)-epigallocatechin, (??)-epicatechin gallate, and (??)-epicatechin were decreased significantly (p?0.05) after TMPF. By using nontargeted metabolomic analysis, a total of 1733 ion features were detected. KEGG pathway enrichment analysis showed that TMPF had a significant impact on caffeine metabolism. Also, theophylline, 3-methylxanthine, and 1,3,7-trimethyluric acid were increased significantly after TMPF, which suggested that demethylation and oxidation reaction might be the main pathways of caffeine metabolism. This study provides a better understanding of the mechanism of TMPF during high-temperature for Chinese dark tea and lays a foundation for further research.
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KEYWORD
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Thermophilic microorganism, High-temperature, Chinese dark tea, Pile-fermentation, Nontargeted metabolomic analysis
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